Unknown Lab Report

foreigner Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 referable Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae unaffectionate from the boundn #103 was a negative Rod. The purpose of this laboratory was to separate and come out the genus and species of an unknown bacterium. It is strategic to identify an unknown micro-organism because cognize how the bacteria work and how it is organise means knowing how it can affect humans. Unknown bacteria may similarly be employ clinically legion(predicate) pharmaceutical drugs argon based on products made by organisms (Katzung, B.G.2004). In ready to identify the unknown organism a series of tests were per make. The tests use were glucose, sucrose, lactose fermentation; Urease, motility, MR-VP, citrate, oxidase, and indole these tests were used to identify the genus of the unknown and a McConkey nutrient agar scale leaf was used to differ entiate and confirm the species of the unknown. This draw will explain and give more in astuteness reasons why certain tests were make and the results yielded from them. Materials & Methods On the first daylight after receiving the unknown micro-organism a streak case was aseptically done as shown in exercise 2 of the cub manual (Kleyn 9).

The streak plate proficiency is done to withdraw a colony formed by a maven cell from a motley containing millions of cells. The streak plate technique was also used to restrain pure cultures of the bacteria victimization a trypticase soy agar (TSA) plate and was incuba ted at 37 ? for 48 hours. Secondly a deoxygu! anosine monophosphate stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the positioning of the cells; if an organism appears purple below a microscope then it is state to be Gram positive, and if it appears criticise then it is said to be Gram negative. To prevent the organism from embark onting contaminated,...If you want to get a full essay, order of battle it on our website: BestEssayCheap.com

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